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recU [2018-07-30 12:24:33]
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recU [2018-07-30 12:24:33]

Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, equivalent to E. coli RecU, required for efficient survival and replication restart after replication-transcription conflicts
Locus
BSU22310
Isoelectric point
9.51
Molecular weight
23.81 kDa
Protein length
206 aa Sequence Blast
Gene length
618 bp Sequence Blast
Function
DNA repair/ recombination and chromosome segregation
Product
Holliday junction resolvase
Essential
no
Synonyms
prfA,yppB,jopB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → DNA repair/ recombination
  • Information processing → Genetics → DNA repair/ recombination → Double strand breaks repair
  • Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)

    • Gene

    Coordinates
    2,340,802 → 2,341,422

    Phenotypes of a mutant

  • the inactivations of recU and ruvA-ruvB are synthetically lethal PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • binds Holliday junctions with high affinity PubMed

    • Paralogous protein(s)

  • Mfd

    • Effectors of protein activity

  • RecU-mediated Holliday junction resolution is stimulated by RuvB-ATP PubMed

    • Structure

  • 1ZP7 PubMed

    • Expression and Regulation

    Operons

    Genes
    recU-ponA
    Description
    PubMed

    Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon
  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulation

  • constitutive during vegetative growth PubMed
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    Biological materials

    Mutant

  • GP891 (recU::cat), available in Jörg Stülke's lab
  • BKE22310 ( recU::ermC), (available at the BGSC and in Fabian Commichau's lab) PubMed
  • BP784 ( recU::ermC), (available in Fabian Commichau's lab)
  • 1A895 (no resistance), PubMed, available at BGSC
  • BKE22310 (ΔrecU::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT, downstream forward: _UP4_TGATTAACGAAAGGTTGAGA
  • BKK22310 (ΔrecU::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT, downstream forward: _UP4_TGATTAACGAAAGGTTGAGA

    • References

    Reviews

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    Original publications

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    RecU in other organisms

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